IMRS (Identical Multi Repeat Sequences) is a novel concept in de-novo mining of pathogen genomes. Unlike other techniques, the approach identifies all possible varying sizes of repeat sequence regions distributed throughout the genome in an very uneven pattern to identify any two close by repeat regions which can be used as primer/probe set. While conventional techniques hybridize/amplify one specific target region, IMRS biomarker primer/probes initiate hybridization/amplification at multiple places. This results in a much larger number of products making the assay 10x-100x sensitive than conventional and, specific, possibility for using direct/ non-invasive clinical samples/ flexibility in developing assays- Amplification/ hybridization.